1
Ated in RNase free Biotinylation Buffer (10 mM Tris, pH 7.4) for 1.5 h with rotation; 2 mg of biotin (EZ-Link Biotin-HPDP, Catalog No. 21341) per 1 mg of RNA was used. RNA was extracted by chloroform/ isoamyl alcohol (24:1) and precipitated with 0.5 M NaCl and isopropanol. After dissolving in water, the RNA was heated to 65 C for 10 min, then placed on ice for 5 min and incubated with streptavidin
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